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Multilayer microstructure of idiopathic epiretinal macular membranes

Multilayer microstructure of idiopathic epiretinal macular membranes

Post author correction

Article Type: ORIGINAL RESEARCH ARTICLE

Article Subject: Vitreo SURG

DOI:10.5301/ejo.5000982

Authors

Claudio Azzolini, Terenzio Congiu, Simone Donati, Alberto Passi, Petra Basso, Eliana Piantanida, Cesare Mariotti, Francesco Testa, Simona M. Caprani, Jennifer Cattaneo, Riccardo Vinciguerra

Abstract

Purpose

To identify the ultramicroscopic structure of idiopathic epiretinal macular membranes (iEMMs) by scanning electron microscopy (SEM).

Methods

We examined 28 iEMMs surgically removed from 28 eyes of 28 patients. All specimens, previously observed at stereomicroscope, were treated with an osmium maceration technique. Fine resolution of iEMMs’ 3D architecture and their interaction with the retina were studied using a Philips SEM-FEG XL-30 microscope.

Results

The specimens appeared as laminar connective structures partially or completely adherent to the inner limiting membrane (ILM). We identified 4 types of structures: (1) distinct layers of thin sheets of woven fibers; (2) folded layers of inhomogeneous thickness of fibrous material more consistent; (3) thicker and more rigid layers recognizable as collagen fibrils with typical 64-nm period, collagen fibrils isolated or intermingled between them; (4) lacunar structures with inflammatory and/or necrotic material. The first 3 types of structures appear to thicken towards a centripetal direction from the ILM to the vitreous in order from 1 to 3. The interface of ILM-iEMM tissue shows particular small bridges of connection. Cells are rarely found, especially in the tissue near the ILM.

Conclusions

Layers of various materials follow one another in iEMMs. Cells are rarely found. The interface ILM-iEMM tissue shows particular small bridges of connection. The dynamic modeling of bended layers begins in soft tissue.

Article History

Disclosures

Financial support: No financial support was received for this submission.
Conflict of interest: None of the authors has conflict of interest with this submission.

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Authors

Affiliations

  • Department of Medicine and Surgery, University of Insubria, Varese - Italy
  • Department of Ophthalmology, Polytechnic University of Ancona, Ancona - Italy
  • Eye Clinic, Multidisciplinary Department of Medical, Surgical and Dental Sciences, Second University of Naples, Naples - Italy

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