Advertisement

Role of dextran in maintaining adhesive and stiffness properties of prestripped DMEK lenticules

Abstract

Purpose

To investigate the adhesive and stiffness properties of prestripped Descemet membrane endothelial keratoplasty (DMEK) lenticules in different preservation conditions (with and without dextran).

Methods

The study included 3 conditions: (C1) tissues collected from tissue culture media (TCM), stripped and preserved in TCM; (C2) tissues collected from transport media (TM) (TCM supplemented with 6% dextran T-500), stripped and preserved in TM; and (C3) tissues collected from TCM, stripped and preserved in TM. Using a hinge, 9.5-mm stripped DMEK lenticules were restored back on the stroma and preserved for 4 days at room temperature (RT) in different conditions as above. Nine tissues, 3 from each condition, were used to check the adhesive (fibronectin, laminin, and vitronectin) and elastic properties (fibrillin, elastin, and collagen VI) using different antibodies. Six tissues, 2 from each condition, were used to check the stiffness properties after preservation using atomic force microscopy (AFM) nanoindentation method.

Results

On the Descemet membrane, fibronectin was strongly expressed in C2 and C3, whereas laminin was intense in C2 postpreservation. Vitronectin was expressed in all the conditions. Elastic proteins were not expressed in either of the conditions apart from collagen VI, which was expressed on the posterior stroma. Atomic force microscopy showed higher stiffness in C3 and an insignificant but lower rigidity in C2 as compared to C1.

Conclusions

The tissues from C2 showed expression of adherent proteins and lower stiffness. Dextran may be suitable in preservation of DMEK grafts before and after preparation. Less stiff tissues may help reduce manipulations required in the recipient eye during DMEK surgery.

Eur J Ophthalmol 2017; 27(3): 270 - 277

Article Type: ORIGINAL RESEARCH ARTICLE

DOI:10.5301/ejo.5000906

Authors

Mohit Parekh, Alessandro Ruzza, Rosa Di Mundo, Stefano Ferrari, Giuseppina Recchia, Hossein Elbadawy, Giuseppe Carbone, Diego Ponzin

Article History

Disclosures

Financial support: Supported by the 2014 Eye Bank Association of America (EBAA) Richard Lindstrom Research Grant and the Società Oftalmologica Italiana (SOI) grant to Dr. Diego Ponzin.
Conflict of interest: None of the authors has conflict of interest with this submission.
Meeting presentation: Derived from a paper presented at the Cornea Society/EBAA Fall Educational Symposium, Las Vegas, Nevada, November 13, 2015.

This article is available as full text PDF.

  • If you are a Subscriber, please log in now.

  • Article price: Eur 36,00
  • You will be granted access to the article for 72 hours and you will be able to download any format (PDF or ePUB). The article will be available in your login area under "My PayPerView". You will need to register a new account (unless you already own an account with this journal), and you will be guided through our online shop. Online purchases are paid by Credit Card through PayPal.
  • If you are not a Subscriber you may:
  • Subscribe to this journal
  • Unlimited access to all our archives, 24 hour a day, every day of the week.

Authors

Affiliations

  • International Center for Ocular Physiopathology, Fondazione Banca degli Occhi del Veneto Onlus, Venice - Italy
  • Tribolab, DMMM, Politecnico di Bari, Bari - Italy
  • Department of Pharmacology and Toxicology, Taibah University, Almadinah Almunawwarah - Saudi Arabia

Article usage statistics

The blue line displays unique views in the time frame indicated.
The yellow line displays unique downloads.
Views and downloads are counted only once per session.

No supplementary material is available for this article.