To evaluate the technical feasibility of isolated Bowman layer (BL) graft preparation by femtosecond laser (FSL) and to compare the ultrastructural morphology to manually dissected grafts.
Five whole globes were placed in custom-made eye holders and debrided of epithelium. After programming a dissection depth of 20 μm, the FSL was docked into position and 5 isolated BL grafts were created. From 5 additional globes, corneoscleral buttons were procured, mounted in artificial anterior chambers, and stripped of BL via the previously described manual technique. Three specimens from both series were randomly selected and assigned to transmission electron microscopy for ultrastructural evaluation and thickness measurements.
All dissections were uneventful and 10 total grafts were produced: 5 by FSL and 5 by manual dissection. Mean graft thickness was 37 (±8.6) μm (n = 3) for the FSL group and 11.7 (±1.6) μm (n = 3) for the manually dissected group. Transmission electron microscopy revealed a thick but relatively smooth posterior cut edge in the FSL group, versus a virtually isolated BL with irregular rests of dispersed stroma in the manually dissected group.
Femtosecond laser may have potential for harvesting intact BL and with a smooth posterior surface, but accompanied by variable amounts of anterior stroma owing to technical limitations.
Eur J Ophthalmol 2017; 27(6): 675 - 677
Article Type: ORIGINAL RESEARCH ARTICLE
AuthorsJack S. Parker, Forest Huls, Elizabeth Cooper, Paul Graves, Esther A. Groeneveld-van Beek, Jessica Lie, Gerrit R.J. Melles
- • Accepted on 08/05/2017
- • Available online on 26/05/2017
- • Published in print on 08/11/2017
This article is available as full text PDF.
- Parker, Jack S. [PubMed] [Google Scholar] 1, 2, 3
- Huls, Forest [PubMed] [Google Scholar] 4
- Cooper, Elizabeth [PubMed] [Google Scholar] 3
- Graves, Paul [PubMed] [Google Scholar] 5
- Groeneveld-van Beek, Esther A. [PubMed] [Google Scholar] 1, 6
- Lie, Jessica [PubMed] [Google Scholar] 1, 6
- Melles, Gerrit R.J. [PubMed] [Google Scholar] 1, 2, 6, * Corresponding Author (firstname.lastname@example.org)
Netherlands Institute for Innovative Ocular Surgery, Rotterdam - The Netherlands
Melles Cornea Clinic Rotterdam, Rotterdam - The Netherlands
Parker Cornea, Birmingham, Alabama - USA
University of Michigan, Ann Arbor, Michigan - USA
Alabama Eye Bank, Birmingham, Alabama - USA
Amnitrans Eye Bank Rotterdam, Rotterdam - The Netherlands