The neovascular or wet form of age-related macular degeneration is characterized by the growth of abnormal blood vessels in the retina stimulated by vascular endothelial growth factors (VEGF). In the last decade, several anti-VEGF drugs have been developed for treating neovascular diseases of the eyes. This study was conducted to compare the effects of 2 anti-VEGF-A drugs, ranibizumab and aflibercept, on the expression and secretion of VEGF family members in retinal pigment epithelial cells (RPE)
ARPE-19 cells were exposed for 24 hours to ranibizumab or aflibercept at clinical dose concentration. Cell viability and expression and secretion of VEGF-A, VEGF-B, VEGF-C, and placental growth factor (PlGF) were evaluated respectively by real-time polymerase chain reaction and enzyme-linked immunosorbent assay.
Ranibizumab and aflibercept did not affect ARPE-19 cell viability after 24 hours of treatment. Ranibizumab increased expression of VEGF-A and PlGF. On the contrary, expression and secretion of VEGF-C was decreased by ranibizumab. PlGF secretion was not affected by ranibizumab. Aflibercept strongly increased VEGF-A and PlGF expression but reduced their detection on the culture media, and decreased expression and secretion of VEGF-C. No effect on expression and secretion of VEGF-B was observed after exposure to these drugs.
Ranibizumab and aflibercept exert similar effects on VEGF expression and secretion, leading to establishing an antiangiogenic environment. Increased VEGF-A expression observed in RPE cells treated with these drugs suggests a compensatory response of the cells to the lack of VEGF-A.
Eur J Ophthalmol 2016; 26(5): 425 - 430
Article Type: ORIGINAL RESEARCH ARTICLE
AuthorsAlessandra Puddu, Roberta Sanguineti, Carlo Enrico Traverso, Giorgio L. Viviani, Massimo Nicolò
- • Accepted on 06/03/2016
- • Available online on 12/04/2016
- • Published in print on 04/08/2016
This article is available as full text PDF.
- Puddu, Alessandra [PubMed] [Google Scholar] 1, * Corresponding Author (firstname.lastname@example.org)
- Sanguineti, Roberta [PubMed] [Google Scholar] 1
- Traverso, Carlo Enrico [PubMed] [Google Scholar] 2
- Viviani, Giorgio L. [PubMed] [Google Scholar] 1
- Nicolò, Massimo [PubMed] [Google Scholar] 2, 3
Department of Internal Medicine and Medical Specialties, Genova - Italy
Department of Neuroscience, Ophthalmology and Genetics, Genova - Italy
Fondazione per la Macula onlus, Genova - Italy